iLite TNF-alpha Assay Ready Cells
TNF-alpha Assay Ready Cells Developed and Manufactured by Svar Life Science
The Eagle Biosciences iLite® TNF-alpha Assay Ready Cells are intended for:
- Testing anti-TNF-alpha activity
- Testing NAb anti-TNF-alpha activity
Content: 1.5 ml of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), 10% glycerol, and 2.5% dimethyl sulfoxide (DMSO) .
Storage: -80°C, Cells should be used within 30 min of thawing.
For Research Use Only
Key benefits of iLite® Assays
- Highly specific reporter gene cell lines
- Very sensitive cell line responses (>10 fold inductions)
- Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
- Assays within a workday (typically 4-7 hour assays)
- Normalization gene, which eliminates unwanted matrix effects
Assay Principle
The iLite™ TNF-alpha Assay Ready Cells are engineered cells optimized to express Firefly luciferase under the control of a TNF-alpha responsive promoter and Renilla luciferase under the control of a constitutive promoter. TNF-alpha binds to the TNF receptor on the cell surface and activates the TNF-alpha regulated Firefly luciferase reporter gene construct. The Firefly luciferase signal can be measured in a luminometer following addition and incubation of the luciferase substrate. The Firefly luciferase signal is proportional to the functional activity of TNF-alpha in the sample. In the presence of TNF-alpha inhibitor activity in test samples, the amount of free TNF-alpha is reduced, resulting in a decreased stimulation of Firefly luciferase production. The Firefly luciferase signal is inversely proportional to the amount of anti-TNF-alpha activity in the samples. The constitutively expressed gene Renilla luciferase can be sequentially measured in the same wells as the Firefly luciferase, following addition and incubation of the Renilla substrate. This allows for normalization of results by compensating for cell number variation and eliminating potential matrix effects from a serum sample, for example. In samples with no suspected matrix effect, the Renilla reading may be excluded.
