TBARS ELISA Assay Kit
The TBARS ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.5 µM
Standard Range: 0.5 – 20 µM
Incubation Time: 30 minutes
Sample Type: Serum, Plasma, Urine
Sample Size: 100 µl
Product Developed and Manufactured in the USA
2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
1. The reagents of the TBARS ELISA are stable until the indicated kit expiration date if handled and stored properly.
2. When not in use, store the TBARS ELISA Assay Kit at 4°C for up to one year.
3. MDA standards should be used within 24 hours of preparation.
4. The Indicator Solution (combined 2-TBA and Acid Solution) can be stored at 4˚C forone week.