T7 RNA Polymerase ELISA Assay Kit


The T7 RNA Polymerase ELISA Assay Kit is designed for the quantitative detection of residual T7 RNA Polymerase content added in RNA pharmaceuticals processes. The T7 RNA Polymerase ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

T7 RNA Polymerase ELISA Assay Kit

The T7 RNA Polymerase ELISA Assay Kit is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 4 – 256 ng/mL
Sample Size: 100 uL
Incubation Time: 3 hours 10 minutes
Sample Type: test samples

Assay Principle

This product uses the double-antibody sandwich method to detect T7 RNA polymerase in samples, which involves coating microplate wells with a monoclonal antibody specific to T7 RNA polymerase, and adding the standard and the test sample to the reaction well for incubation. T7 RNA Polymerase in the test sample will quantitatively bind to the antibody in the microplate, and the test procedure is to remove the unbound complex by washing, add the antibody against T7 RNA Polymerase (detection antibody), and finally add H + L secondary antibody (enzyme conjugate) to form an antibody-antigen-antibody-secondary antibody complex, and indicate the protein content in the sample by observing the intensity of TMB color development. Please read the instructions for use carefully and check the components of the kit before use.

Storage conditions and shelf life

The shelf life is 12 months when stored at 2-8℃ and below.

Applicable models (including but not limited to)

  • ABI QuantStudio 3 qPCR System
  • Roche LC96 Real-Time PCR System
  • ABI 7500 Real-Time PCR System
  • Bio-Rad CFX Opus96 Real-Time PCR System
  • RocGene ArchimedTM X Real-Time PCR System

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Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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