Benzonase Nuclease ELISA Assay Kit


The Benzonase Nuclease ELISA Assay Kit is designed for the quantitative detection of residual nuclease content in intermediates, semi-finished products and finished products of various biological products. The Benzonase Nuclease ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Benzonase Nuclease ELISA Assay Kit

The Benzonase Nuclease ELISA Assay Kit is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 0.05 ng/mL
Dynamic Range: 0.05 – 5 ng/mL
Sample Size: 100 uL
Incubation Time: 2 hours 15 minutes
Sample Type: intermediates, semi-finished products, finished products of various biological products

Assay Principle

The kit can quantitative detect trace residual Benzonase nuclease (hereinafter referred to as “nuclease”) in test samples by using a double-antibody sandwich method. Coat the 96-well plate with capture antibody to prepare immobilized antibodies. Add the standard and test samples, and then horseradish peroxidase (HRP) conjugated antibody to form an immobilized antibody – nuclease – conjugated antibody sandwich conjugate. Wash the plate after reaction, and add the substrate for color development reaction. The substrate will turn blue under HRP catalysis, and will finally turn yellow under the action of the stop solution. Determine the optical density (OD) value at 450 nm, and calculate the nuclease content in the test sample using the standard curve.

Storage conditions and shelf life

The shelf life is 12 months when stored at 2-8℃ and below.

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Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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