T7 RNA Polymerase ELISA Assay Kit
The T7 RNA Polymerase ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 4 – 256 ng/mL
Sample Size: 100 uL
Incubation Time: 3 hours 10 minutes
Sample Type: test samples
Assay Principle
This product uses the double-antibody sandwich method to detect T7 RNA polymerase in samples, which involves coating microplate wells with a monoclonal antibody specific to T7 RNA polymerase, and adding the standard and the test sample to the reaction well for incubation. T7 RNA Polymerase in the test sample will quantitatively bind to the antibody in the microplate, and the test procedure is to remove the unbound complex by washing, add the antibody against T7 RNA Polymerase (detection antibody), and finally add H + L secondary antibody (enzyme conjugate) to form an antibody-antigen-antibody-secondary antibody complex, and indicate the protein content in the sample by observing the intensity of TMB color development. Please read the instructions for use carefully and check the components of the kit before use.
Storage conditions and shelf life
The shelf life is 12 months when stored at 2-8℃ and below.
Applicable models (including but not limited to)
- ABI QuantStudio 3 qPCR System
- Roche LC96 Real-Time PCR System
- ABI 7500 Real-Time PCR System
- Bio-Rad CFX Opus96 Real-Time PCR System
- RocGene ArchimedTM X Real-Time PCR System
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