Soluble Leptin Receptor ELISA

$560.00

The Soluble Leptin Receptor (sLEP-R) ELISA Assay Kit is intended for the quantitative measurement of soluble human leptin receptor (sLEP-R) in human serum or plasma for research purposes. The Eagle Biosciences Soluble Leptin Receptor (sLEP-R) ELISA Assay Kit is for research use only and not intended for use in diagnostic procedures.

Soluble Leptin Receptor ELISA

The Soluble Leptin Receptor ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.0385 ng/mL
Standard Range: 0.0385 – 150 ng/mL
Incubation Time: 5 hours
Sample Type: Serum or Plasma
Sample Size: 30 µL
Controls Included

Product is manufactured by Mediagnost


Assay Background

The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. The amount of functionally active LEP-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sLEP-R, soluble Leptin receptor), is the main binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sLEP-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity and can therefore enhance or reduce leptin sensitivity. Lipotoxicity and apoptosis increase LEP-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased sLEP-R concentrations seem directly to inhibit leptin effects, reduced amounts of sLEP-R may reflect decreased membrane expression of LEP-R. These findings, in part, explain alterations of leptin sensitivity that are associated with changes in serum sLEP-R concentrations seen in metabolic disorders.


Sample stability
In firmly closable sample vials:
• Storage at Room Temperature 20-25°C: max. 2 days
• Storage at 4°C: max. 3 days
• Storage at -20°C: 2 years
• Freeze/Thaw cycles: max. 3
Freeze-Thaw cycles should be minimized. Up to 3 cycles showed no effect on the measured sLEP-R concentration.
Use standard venipuncture for the blood sampling. Haemolytic reactions have to be avoided.


Related Products

Functional Leptin ELISA Assay
Leptin Sensitive ELISA Assay
Rodent Leptin ELISA Assay

Additional Information

Assay Principle


This ELISA for Soluble Leptin Receptor (sLEP-R) is a so-called Sandwich-Assay using two specific and highly affine antibodies. The sLEP-R in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-sLEP-R-antibody binds in turn to the immobilised sLEP-R. This is biotinylated and allows the binding of a streptavidin-peroxidase enzyme conjugate. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the sLEP-R content of the sample. The reaction is stopped by the addition of stop solution. After stopping the reaction, the color intensity (then yellow) is quantified by measuring the absorbance and converted to the sLEP-R concentration using a standard curve.

Manual

Product Manual