Soluble Leptin Receptor ELISA
The Soluble Leptin Receptor ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.0385 ng/mL
Standard Range: 0.0385 – 150 ng/mL
Incubation Time: 5 hours
Sample Type: Serum or Plasma
Sample Size: 30 µL
Product is manufactured by Mediagnost
This ELISA for Soluble Leptin Receptor (sLEP-R) is a so-called Sandwich-Assay using two specific and highly affine antibodies. The sLEP-R in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-sLEP-R-antibody binds in turn to the immobilised sLEP-R. This is biotinylated and allows the binding of a streptavidin-peroxidase enzyme conjugate. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the sLEP-R content of the sample. The reaction is stopped by the addition of stop solution. After stopping the reaction, the color intensity (then yellow) is quantified by measuring the absorbance and converted to the sLEP-R concentration using a standard curve.
In firmly closable sample vials:
• Storage at Room Temperature 20-25°C: max. 2 days
• Storage at 4°C: max. 3 days
• Storage at -20°C: 2 years
• Freeze/Thaw cycles: max. 3
Freeze-Thaw cycles should be minimized. Up to 3 cycles showed no effect on the measured sLEP-R concentration.
Use standard venipuncture for the blood sampling. Haemolytic reactions have to be avoided.