SeroMP Recombinant IgM ELISA Assay
The SeroMP Recombinant IgM ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µl
Alternative Name: Mycoplasma pneumoniae
The Eagle Biosciences SeroMP Recombinant microtiter plates are supplied coated with purified fraction of M. pneumoniae membrane proteins and recombinant antigens. The serum to be tested is diluted and incubated in the SeroMP Recombinant plate. In this step M. pneumoniae specific antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgM conjugated to horseradish peroxidase (HRP) is added. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the levels of the specific antibodies that are bound to the coated antigens.
Hospitalized patients, infected with respiratory tract pathogens: Chlamydia pneumoniae and EBV who were diagnosed by commercial serology kits, were also tested with the SeroMP kit. Most of the sera were found negative, there was no significant cross-reaction detected.