SeroCT C. trachomatis IgG ELISA Assay
The SeroCT C. trachomatis IgG ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2.25 hours
Sample Type: Serum
Sample Size: 10 µl
Alternative Name: Chlamydia trachomatis
The Eagle Biosciences SeroCT plates are coated with C. trachomatis specific peptides. Serum to be tested is diluted and incubated with the precoated SeroCT plate 1h at 37°C. In this step C. trachomatis specific antibodies are bound to the immobilized C. trachomatis specific peptides. Non specific antibodies are removed by washing. Anti-human IgG conjugated to horseradish peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized peptides.
For the test to be valid the following criteria must be met. If these criteria are not met the test should be considered invalid and should be repeated.
- Positive Control: The absorbance value should be ≥ 0.8 at 450nm.
- Negative Control: The average absorbance value of the Negative Control performed in duplicate should be 0.1< NC ≤ 0.4 at 450nm.