SARS-CoV-2 Surrogate Virus Neutralization Antibody ELISA


The SARS-CoV-2 Surrogate Virus Neutralization Antibody ELISA is an Enzyme Immunoassay for qualitative and quantitative detection all types of neutralization antibodies against SARS-CoV-2 in human serum or plasma. This product is intended for research use only and not for use in diagnostic or clinical procedures.

SARS-CoV-2 Surrogate Virus Neutralization Antibody ELISA

The SARS-CoV-2 Surrogate Virus Neutralization Antibody ELISA is For Research Use Only

Size: 12×8 wells
Sensitivity: uses cutoff with Positive and Negative Control
Incubation Time: 3 hours
Sample Type: Serum, plasma
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 RBD. Samples and controls are pipetted in a blank microtitre plate and incubated with HRP conjugated human SARSCoV-2 RBD protein. The antibodies to SARS-CoV-2 present in the samples and controls bind to the SARSCoV-2 RBD protein to form a complex. This solution of bound and unbound antibodies to SARS-CoV-2 is then pipetted into human ACE2 coated microplate. After washing to remove the bound complex of Anti-SARS-CoV-2 and HRP conjugated SARSCoV-2 RBD, the substrate solution (TMB) is added to the microwells. Post incubation, color develops proportionally to the amount of unbound Anti-SARS-CoV-2 (Covid-19) present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided.
Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted less or more respectively with Sample Diluent accordingly.
The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay.

Related Products

Anti-SARS-CoV-2 Spike Protein IgG Quantitative ELISA
SARS-CoV-2 Spike Protein Antigen Quantitative ELISA
SARS-CoV-2 Spike RBD Antigen Quantitative ELISA

Additional Information

Assay Background

The GENLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a blocking ELISA technique which mimics the virus neutralization process. SARS-CoV-2-neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface that mediate entry into host cells. The S protein has two functional subunits that mediate cell attachment (the S1 subunit, existing of four core domains S1A through S1D) and fusion of the viral and cellular membrane (the S2 subunit). Potent neutralizing antibodies often target the receptor interaction site in S1, disabling receptor interactions. The spike proteins of SARS-CoV-2 commonly bind to the human angiotensin coverting enzyme 2 (ACE2) protein as a host receptor through their S1B domain. Receptor interaction is known to trigger irreversible conformational changes in coronavirus spike proteins enabling membrane fusion. SARS-CoV-2 initiates an immune response, which leads to the production of antibodies. These neutralizing antibodies provide protection against future infection from SARS-CoV-2, as they remain in the circulatory system for months to years post infection.

Package Inserts

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