SARS-CoV-2 Spike S1 Protein Antigen Quantitative ELISA

$590.00

The SARS-CoV-2 Spike Protein Antigen Quantitative ELISA is a method for the quantitative determination of SARS-CoV-2 spike protein Antigen in serum and plasma and cell culture supernatant. The SARS-CoV-2 Spike Protein Antigen Quantitative ELISA is intended for research use only and not for use in diagnostic or clinical procedures.

SARS-CoV-2 Spike S1 Protein Antigen Quantitative ELISA

The SARS-CoV-2 Spike S1 Protein Antigen Quantitative ELISA is For Research Use Only

Size: 12×8 wells
Sensitivity: 6.8 ng/ml
Standard Range: 0- 500 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, cell culture supernatant and plasma
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19


Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.


Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided.
Samples should be diluted 1:5000 (v/v) for optimal recovery, (for example 1 ul sample + 4999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay
Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay.
Note: The sample should be diluted to within the working range of the assay in 1X Assay Diluent. The exact dilution must be determined based on the concentration of specific target in individual samples.


Related Products

Anti-SARS-CoV-2 Spike Protein IgG Qualitative ELISA
Anti-SARS-CoV-2 Spike Protein IgG Quantitative ELISA
Anti-SARS-CoV-2 IgM Spike Protein ELISA

Additional Information

Assay Background


Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike proteins present in the sample are bound by the capture antibodies.

SARS-CoV-2, a single stranded RNA virus, has quickly become the subject of much research worldwide. Coronaviruses are composed of four primary proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid (N). The spike protein (S-protein) performs two primary tasks that aid in host infection: 1) mediates the attachment between the virus and host cell surface receptors such as ACE2, and 2) facilitates viral entry into the host cell by assisting in the fusion of the viral and host cell membranes.

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