Anti-SARS-CoV-2 IgM Spike Protein ELISA
The Anti-SARS-CoV-2 IgM Spike Protein ELISA is For Research Use Only
Size: 12×8 wells
Incubation Time: 3 hours 20 mins
Sample Type: Serum, cell culture supernatant and plasma
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19
The method employs sandwich ELISA technique. Human SARS-CoV-2 protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and IgM Antibodies to human SARS-CoV-2 (Covid-19) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by HRP-conjugated Detection Antigen is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of IgM Anti-Human SARS-CoV-2 (Covid-19) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage:
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
1. Extract as soon as possible after specimen collection as per relevant procedure. The samples should be tested as soon as possible after the extraction. Alternately the extracted samples can be kept in -20°C. Avoid repeated freeze-thaw cycles.
2. Serum- Coagulate at room temperature for 10-20 minutes; centrifuge for 20-min at 2000-3000 rpm. Remove the supernatant. If precipitation appears, recentrifuge.
3. Respiratory Specimens- Collect sample in a sterile container. Centrifuge for 20-mins at 2000-3000 rpm. Remove the supernatant carefully. It is recommended to follow the CDC (Centre for Disease Control), Atlanta, USA guidelines for specimen handling and treatment. (https://www.cdc.gov/urdo/downloads/SpecCollectionGuidelines.pdf)
Note: Grossly hemolyzed samples are not suitable for use in this assay.
The antigens used in the kit for capture and detection are recombinant spike proteins for SARS-CoV-2 (Covid19). The positive control used is a specific monoclonal antibody towards the spike protein of SARS-CoV-2 (Covid-19).