SARS-CoV-2 Neutralizing Antibodies Against Spike Mutation D614G ELISA Assay Kit
The SARS-CoV-2 Neutralizing Antibodies Against Spike Mutation D614G ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Incubation Time: 3 hrs
Sample Type: Serum and plasma
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19
The method employs sandwich ELISA technique. The protein-protein interaction between HRP-D614G and hACE2 can be blocked by neutralizing antibodies against D614G. Samples and controls are pipetted in a blank microtitre plate and incubated with HRP conjugated human SARS-CoV-2 spike mutation D614G protein. The antibodies to SARS-CoV-2 spike mutation D614G present in the samples and controls bind to the SARS-CoV-2 spike mutation D614G protein to form a complex. This solution of bound and unbound antibodies to SARS-CoV-2 spike mutation D614G is then pipetted into human ACE2 coated microplate. After washing to remove the unbound complex and proteins, the substrate solution (TMB) is added to the microwells. Post incubation, color develops proportionally to the amount of Antibodies to SARS-CoV-2 (Covid-19) spike mutation D614G present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay