Bovine SAA ELISA Assay Kit

$540.00

The Eagle Biosciences Bovine SAA ELISA Assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in Bovine biological samples. The Eagle Biosciences Bovine SAA ELISA Assay kit is for research use only and not to be used for diagnostic purposes.

SKU: BSA61-K01 Categories: , ,

Bovine SAA ELISA Assay Kit

The Bovine SAA ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Dynamic Range: 26.34 ng/ml – 300 ng/ml
Incubation Time: 1 hr 40 mins
Sample Type: Plasma, Serum, milk
Sample Size: 100 μL
Alternative Names: Serum Amyloid A


Assay Principle

In this assay the Serum Amyloid A (SAA) present in samples reacts with the anti-SAA antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


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Additional Information

Standard Curve


Bovine SAA ELISA Assay Kit Standard Curve

Documents

Product Manual