SARS-CoV-2 (Covid-19) Beta Neutralizing Antibody (B.1.351, B.1.351.2,B.1.351.3) ELISA Assay Kit
The SARS-CoV-2 (Covid-19) Beta Neutralizing Antibody (B.1.351, B.1.351.2,B.1.351.3) ELISA Assay Kit is For Research Use Only
Size: 96 wells
Standard Range: 15.625 – 2500 ng/mL
Incubation Time: 3 Hours
Sample Type: Cell Culture Supernatant, Serum, Plasma
Sample Size: 100 µL
Assay Principle
The method employs sandwich ELISA technique. The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 RBD (K417N, E484K, N501Y). Samples and controls are pipetted in a blank microtitre plate and incubated with HRP conjugated human SARSCoV-2 RBD (K417N, E484K, N501Y) protein. The antibodies to SARS-CoV-2 (K417N, E484K, N501Y) present in the samples and controls bind to the SARS-CoV-2 RBD (K417N, E484K, N501Y) protein to form a complex. This solution of bound and unbound antibodies to SARS-CoV-2 Variant B.1.351 (Beta) is then pipetted into human ACE2 coated microplate. After washing to remove the bound complex of Anti-SARS-CoV-2 Variant B.1.351, B.1.351.2, B.1.351.3 (Beta) and HRP conjugated SARS-CoV-2 RBD (K417N, E484K, N501Y), the substrate solution (TMB) is added to the microwells. Post incubation, color develops proportionally to the amount of unbound Anti-SARS-CoV-2 (Covid-19) Variant B.1.351, B.1.351.2, B.1.351.3 (Beta) present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided.
Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted less or more respectively with Sample Diluent accordingly.
The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay.
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SARS-CoV-2 (2019-nCoV) Spike RBD Antigen Quantitative ELISA Assay Kit
Assay Background
The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a blocking ELISA technique which mimics the virus neutralization process. rnSARS-CoV-2-neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface that mediate entry into host cells. The S protein has two functional subunits that mediate cell attachment (the S1 subunit, existing of four core domains S1A through S1D) and fusion of the viral and cellular membrane (the S2 subunit). rnPotent neutralizing antibodies often target the receptor interaction site in S1, disabling receptor interactions. The spike proteins of SARS-CoV-2 commonly bind to the human angiotensin coverting enzyme 2 (ACE2) protein as a host receptor through their S1B domain. Receptor interaction is known to trigger irreversible conformational changes in coronavirus spike proteins enabling membrane fusion. rnSARS-CoV-2 initiates an immune response, which leads to the production of antibodies. These neutralizing antibodies provide protection against future infection from SARS-CoV-2 (Variant B.1.351), as they remain in the circulatory system for months to years post infection. rnSARS-CoV-2 keeps evolving by continual mutation which enable the virus to evade vaccines and immune systems. Some of the mutations ,the U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351 and the Indian variant B.1.617, may have allowed the virus to escape from neutralizing antibodies.rnThe combination of K417N, E484K, and N501Y substitutions is present in B.1.351 (Beta) which was first reported in South Africa. rnThis Neutralizing Kit works on using Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD (K417N, E484K, N501Y) Protein and a Neutralizing Antibody sensitive to the variant as a Standard/Calibrator to measure the effective inhibition. The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a blocking ELISA technique which mimics the virus neutralization process. SARS-CoV-2-neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface that mediate entry into host cells. The S protein has two functional subunits that mediate cell attachment (the S1 subunit, existing of four core domains S1A through S1D) and fusion of the viral and cellular membrane (the S2 subunit).
The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a blocking ELISA technique which mimics the virus neutralization process. SARS-CoV-2-neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface that mediate entry into host cells. The S protein has two functional subunits that mediate cell attachment (the S1 subunit, existing of four core domains S1A through S1D) and fusion of the viral and cellular membrane (the S2 subunit). rnPotent neutralizing antibodies often target the receptor interaction site in S1, disabling receptor interactions. The spike proteins of SARS-CoV-2 commonly bind to the human angiotensin coverting enzyme 2 (ACE2) protein as a host receptor through their S1B domain. Receptor interaction is known to trigger irreversible conformational changes in coronavirus spike proteins enabling membrane fusion. rnSARS-CoV-2 initiates an immune response, which leads to the production of antibodies. These neutralizing antibodies provide protection against future infection from SARS-CoV-2 (Variant B.1.351), as they remain in the circulatory system for months to years post infection. rnSARS-CoV-2 keeps evolving by continual mutation which enable the virus to evade vaccines and immune systems. Some of the mutations ,the U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351 and the Indian variant B.1.617, may have allowed the virus to escape from neutralizing antibodies.rnThe combination of K417N, E484K, and N501Y substitutions is present in B.1.351 (Beta) which was first reported in South Africa. rnThis Neutralizing Kit works on using Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD (K417N, E484K, N501Y) Protein and a Neutralizing Antibody sensitive to the variant as a Standard/Calibrator to measure the effective inhibition. Potent neutralizing antibodies often target the receptor interaction site in S1, disabling receptor interactions. The spike proteins of SARS-CoV-2 commonly bind to the human angiotensin coverting enzyme 2 (ACE2) protein as a host receptor through their S1B domain. Receptor interaction is known to trigger irreversible conformational changes in coronavirus spike proteins enabling membrane fusion.
SARS-CoV-2 initiates an immune response, which leads to the production of antibodies. These neutralizing antibodies provide protection against future infection from SARS-CoV-2 (Variant B.1.351), as they remain in the circulatory system for months to years post infection.
SARS-CoV-2 keeps evolving by continual mutation which enable the virus to evade vaccines and immune systems. Some of the mutations ,the U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351 and the Indian variant B.1.617, may have allowed the virus to escape from neutralizing antibodies.
The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a blocking ELISA technique which mimics the virus neutralization process. SARS-CoV-2-neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface that mediate entry into host cells. The S protein has two functional subunits that mediate cell attachment (the S1 subunit, existing of four core domains S1A through S1D) and fusion of the viral and cellular membrane (the S2 subunit). Potent neutralizing antibodies often target the receptor interaction site in S1, disabling receptor interactions. The spike proteins of SARS-CoV-2 commonly bind to the human angiotensin coverting enzyme 2 (ACE2) protein as a host receptor through their S1B domain. Receptor interaction is known to trigger irreversible conformational changes in coronavirus spike proteins enabling membrane fusion. SARS-CoV-2 initiates an immune response, which leads to the production of antibodies. These neutralizing antibodies provide protection against future infection from SARS-CoV-2 (Variant B.1.351), as they remain in the circulatory system for months to years post infection. rnSARS-CoV-2 keeps evolving by continual mutation which enable the virus to evade vaccines and immune systems. Some of the mutations ,the U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351 and the Indian variant B.1.617, may have allowed the virus to escape from neutralizing antibodies. The combination of K417N, E484K, and N501Y substitutions is present in B.1.351 (Beta) which was first reported in South Africa. This Neutralizing Kit works on using Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD (K417N, E484K, N501Y) Protein and a Neutralizing Antibody sensitive to the variant as a Standard/Calibrator to measure the effective inhibition. The combination of K417N, E484K, and N501Y substitutions is present in B.1.351 (Beta) which was first reported in South Africa.
This Neutralizing Kit works on using Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD (K417N, E484K, N501Y) Protein and a Neutralizing Antibody sensitive to the variant as a Standard/Calibrator to measure the effective inhibition %.
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