Rat VEGF ELISA Assay Kit

$490.00

The Eagle Biosciences Rat VEGF ELISA Assay Kit is intended for the quantitative measurement of VEGF in rat serum/plasma, urine, and more. The Rat VEGF ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: VGF21-K01 Categories: , ,

Rat VEGF ELISA Assay Kit

The Rat VEGF ELISA Assay Kit is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 2.60 pg/mL (50 uL of Sample) 3.77 pg/mL (10 uL of Sample)
Standard Range: 6.86 – 5000 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Urine, and other sample types
Sample Size: 50 uL or 10 uL
Alternative Names: Vascular endothelial growth factor


Standard Curve Preparation:

S1 to S7 and S0 is ready to use for serum and plasma.

For other sample types, prepare the standard curve with whatever buffer (SPB, Sample Prepared Buffer) is used to prepare the sample, such as cell culture supernatant, tissue grinding liquid, cell lysate, etc. For urine samples use AB (Assay Buffer) to prepare the standard curve.

The Rat VEGF Standard 50,000 pg/mL 30 µL + 270 µL SPB serves as the high standard (5,000 pg/mL). Pipette 200 µL of SPB into each tube. Use the high standard to produce a 1:2 dilution series. Mix each tube thoroughly before the next transfer. SPB serves as the zero standard (0 pg/mL).

Note: After the kit is opened, the stabilization period of each content is 30 days, so please use the kit within 30 days after opening.


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Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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