Rat Urocortin 2 ELISA Assay
The Rat Urocortin 2 ELISA Assay is For Research Use Only
Size: 1×96 wells
Dynamic Range: 1.563 – 100 ng/mL
Incubation Time: 21 hours
Sample Type: Rat Serum, Plasma
Sample Size: 50 µL
Alternative Names: UCN 2, Stresscopin-related Peptide
Stability and Storage
Storage: Store all the components at 2~8°C.
Shelf life: The kit is stable under the condition for 15 months from the date of manufacturing. The expiry date is stated on the package.
Package: For 96 tests per one kit including standards.
Eagle Biosciences Rat Urocortin 2 ELISA for determination of rat urocortin 2 in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat urocortin 2 and biotin-avidin affinity system. To the wells of plate coated with rabbit anti rat urocortin 2 antibody, standards or samples and labeled antigen (biotinylated antigen) are added for competitive immunoreaction. After incubation and plate washing, horseradish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-labeled antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of rat urocortin 2 is calculated.