High Sensitive IFN gamma ELISA Assay

Rat Prorenin & Renin ELISA Assay

$1,080.00

The Rat Prorenin & Renin ELISA Assay kit is for the quantitative determination of Rat Prorenin and Renin level in biological fluids by an ELISA (Enzyme-Linked Immunosorbent Assay). The Rat Prorenin & Renin ELISA Assay is for research use only and not intended for use in diagnostic procedures.

SKU: PRO29-K01 Categories: ,

Rat Prorenin & Renin ELISA Assay

The Rat Prorenin & Renin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.1 – 100 ng/mL
Incubation Time: 1.5 hour
Sample Type: Biological Fluids
Sample Size: 100 µl

Product manufactured in the USA

Reference Values (Normal)
Rat prorenin levels range from 0-400 ng/ml depending on assay methodology. Human plasma levels of prorenin are greater in males than females and correlate positively with age and negatively with blood pressure. Plasma and serum concentrations increase in several conditions such as pregnancy, progressive diabetes mellitus, diabetes mellitus with microvascular disease, and diabetic retinopathy.

It is suggested that each laboratory establish its own normal ranges.

Assay Background

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.

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Additional Information

Assay Principle

This Rat Prorenin / Renin ELISA Assay Kit uses a capture antibody coated to the 96-well plate to bind Rat Prorenin and Renin. A detection antibody conjugated to biotin is then applied. After washing, avidin conjugated to horseradish peroxidase (HRP) is applied. TMB substrate is added for color development which is proportional to the total concentration of prorenin and renin in the sample. Sample concentrations can be determined by comparing OD values to the standard curve.

  • Add 100 µl of the Standards and unknowns to the wells in duplicate. Shake the plate at 300 rpm for 30 minutes at room temperature (RT).
  • Wash the plate 3 times according to the following wash procedure:
    • Remove the contents of each well by inversion of the plate.
    • Tap out the remaining contents of the plate onto a lint free paper towel.
    • Add 300 µL of 1x Wash Buffer.
    • Let stand for 2-3 minutes.
    • Repeat procedure two more times, then proceed to next step.
    • Remove the contents of each well by inversion of plate into an appropriate disposal device.
    • Tap out the remaining contents of the plate onto a lint-free paper towel, then proceed to step 3.
    • Add 100 µl of the Primary Antibody to each well.  Shake the plate at 300 rpm for 30 minutes at RT.
  • Wash the plate three times as in step 2.
  • Add 100 µl of the Secondary Antibody to each well. Shake the plate at 300rpm for 30 minutes at RT.
  • Wash the plate three times as in step 2.
  • Add 100 µl of TMB Substrate to each well. Shake the plate at 300 rpm for 2-10 minutes at RT.
  • Stop the reaction by adding 50 µl of 1N H2SO4 to each well and read the plate at 450 nm.

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