Rat KIM-1 ELISA Assay

$580.00

The Rat KIM-1 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological samples of rats.

Rat KIM-1 ELISA Assay

The Rat KIM-1 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.039 ng/ml
Dynamic Range: 0.1 ng/ml – 10 ng/ml
Incubation Time: 170 minutes
Sample Type: Plasma, Serum, Urine
Alternative Names: Kidney injury molecule 1, KIM 1
Sample Size: 100 μL


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound KIM-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. For urine samples, collect using standard techniques. The samples should be centrifuged to remove any possible debris. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.


Related Products

Mouse KIM-1 ELISA Assay Kit
Human KIM-1 ELISA Kit
Canine (Dog) KIM-1 ELISA Assay Kit

Additional Information

Assay Background


KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.

Typical Standard Curve


Manual

Product Manual