Canine (Dog) KIM-1 ELISA Assay Kit

$510.00

The Eagle Biosciences Canine (Dog) Kim-1 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological fluids of Dogs.

Canine (Dog) KIM-1 ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.034 ng/ml
Dynamic Range: 0.03125 ng/ml – 1 ng/ml
Incubation Time: 210 minutes
Sample Type: Plasma, Serum, Urine
Sample Size: 100 μL

Product manufactured in the U.S.A

Additional Information

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed f rom the standards, and corrected for sample dilution.

Typical Standard Curve


Manual

Product Manual