Rat IL-1 beta ELISA Assay Kit
The Rat IL-1 beta ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 1.46 pg/mL
Standard Range: 13.72 – 10000 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Urine, and other sample types
Sample Size: 10 uL
Alternative Names: Interleukin 18
Standard Curve Preparation:
S1 to S7 and S0 is ready to use for serum and plasma.
For other sample types, prepare the standard curve with whatever buffer (SPB, Sample Prepared Buffer) is used to prepare the sample, such as cell culture supernatant, tissue grinding liquid, cell lysate, etc. For urine samples use AB (Assay Buffer) to prepare the standard curve.
The Rat IL-1 beta Standard 80,000 pg/mL 30 µL + 270 µL SPB serves as the high standard (8,000 pg/mL). Pipette 200 µL of SPB into each tube. Use the high standard to produce a 1:2 dilution series. Mix each tube thoroughly before the next transfer. SPB serves as the zero standard (0 pg/mL).
Note: After the kit is opened, the stabilization period of each content is 30 days, so please use the kit within 30 days after opening.
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