Neutrophil Elastase (NE) ELISA Assay Kit

$640.00

The Neutrophil Elastase (NE) ELISA Assay Kit is designed for quantification of human in serum and plasma samples. The Eagle Biosciences Neutrophil Elastase (NE) ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

Neutrophil Elastase (NE) ELISA Assay Kit

The Neutrophil Elastase (NE) ELISA Assay Kit is For Research Use Only
YES
Size: 1×96 wells
Sensitivity: 0.156ng/mL
Standard Range: 0.156g/mL-10ng/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: NE, leukocyte elastase, serine elastase, and elaszym


Assay Principle

The Neutrophil Elastase (NE) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for human NE. Standards and samples are pipetted into the wells and any human NE present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for human NE is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human NE bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human NE, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.

Sample Preparation

Serum or plasma sample is generally required a 100-fold dilution in the 1×Assay buffer. A suggested dilution step is to add 10 µl of sample to 990µl of 1×Assay buffer. If a sample has a NE level greater than the highest standard, the sample should be diluted further and the assay should be repeated.


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Additional Information

Assay Background


Neutrophil elastase (NE), also known as leukocyte elastase, serine elastase, and elaszym, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs). The primary function of NE is recognized as to participate in the clearance of invaded pathogens through its intracellular and extracellular killing as well as antimicrobial activity, and the degradation of extracellular matrix components, including elastin, collagens, fibroncetin and proteoglycans. Accumulated evidence has also demonstrated that NE can regulate inflammatory process through promoting chemokine and cytokine activation and degradation, cytokine receptor shedding, proteolysis of cytokine binding proteins and the activation of different specific cell surface receptors. The NE activity is strictly controlled by a set of associated endogenous inhibitors, such as the alpha-1 antitrypsin (A1AT) and elafin. Once they escape from the exact regulation, neutrophil serine proteases become invasive and destructive within a human body thereby contributing to a wide range of pathologies, such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, atherosclerosis, diabetes mellitus.

Typical Standard Curve

Neutrophil Elastase ELISA Kit

Documents

Product Manual


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