Neutrophil Elastase (NE) ELISA Assay Kit
The Neutrophil Elastase (NE) ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Standard Range: 0.156g/mL-10ng/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: NE, leukocyte elastase, serine elastase, and elaszym
The Neutrophil Elastase (NE) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for human NE. Standards and samples are pipetted into the wells and any human NE present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for human NE is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human NE bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human NE, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.
Serum or plasma sample is generally required a 100-fold dilution in the 1×Assay buffer. A suggested dilution step is to add 10 µl of sample to 990µl of 1×Assay buffer. If a sample has a NE level greater than the highest standard, the sample should be diluted further and the assay should be repeated.