Mouse tPA Total ELISA Assay

$1,005.00

The Mouse tPA Total ELISA Assay is for the quantitative determination of total tPA in mouse biological fluids by an ELISA assay (Enzyme-Linked Immunosorbent Assay). The Mouse tPA Total ELISA Assay is for research use only and not for use in diagnostic procedures.

Mouse tPA Total ELISA Assay

The Mouse tPA Total ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.1 – 50 ng/ml
Incubation Time: 2 hours
Sample Type: Biological Fluids
Sample Size: 100 µl
Alternative Name: Tissue Type Plasminogen Activator
Product manufactured in the USA


Reference Values (Normal)

The concentration level of tPA antigen in mouse plasma has been reported to be 2.5+/-1.0 ng/ml. In-house testing of pooled normal mouse plasma in citrate indicates tPA levels vary by mouse strain.

Strain Active tPA Total tPA
NSA/CF-1 9.9 ng/mL 9.4 ng/mL
C57BL6 1.4 ng/mL 2.4 ng/mL
CD-1 0.4 ng/mL 0.4 ng/mL

It is suggested that each laboratory establish its own normal ranges


Assay Background

The Mouse Tissue-type Plasminogen Activator (tPA) Total Antigen ELISA assay kit is intended for the quantitative determination of total plasminogen activator antigen in biological fluids. Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to the active serine protease plasmin in the blood fibrinolytic system. It also plays an important role in the removal of incipient thrombi. tPA is widely used for the thrombolytic treatment of acute myocardial infarction.


Related Products

Mouse tPA Active ELISA Assay
Human tPA Total ELISA Assay
Rat tPA Total ELISA Assay

Additional Information

Assay Principle


In the Mouse Tissue-type Plasminogen Activator (tPA) Total Antigen ELISA Assay kit, mouse tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm.

  1. Add 100 µl of the Standards and unknowns to the wells in duplicate. Shake the plate at 300 rpm for 30 minutes at room temperature (RT). See Scheme 1 below for a sample plate layout.
  2. Wash the plate three times with 300 µL of Wash Buffer.  Remove excess Wash Buffer by gently tapping the plate on a paper towel.
  3. Add 100 ml of the Primary Antibody to each well. Shake the plate at 300 rpm for 30 minutes at RT.
  4. Wash the plate three times with 300 µL of Wash Buffer as in step 2.
  5. Add 100 ml of the Primary Antibody to each well. Shake the plate at 300 rpm for 30 minutes at RT.
  6. Wash the plate three times with 300 µL of Wash Buffer as in step 2.
  7. Add 100 µl of TMB Substrate to each well. Shake the plate at 300 rpm for 2-10 minutes at RT.
  8. Stop the reaction by adding 50 µl of 1N H2SO4 and read the plate at 450 nm.

Manual

Product Manual


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