Human tPA Total ELISA Assay
The Human tPA Total ELISA Assay is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 0.1 ng/ml
Dynamic Range: 0.1 – 10 ng/ml
Incubation Time: 2 hours
Sample Type: Biological Fluids
Sample Size: 100 µl
Alternative Name: Tissue-type Plasminogen Activator
Product Developed and Manufactured in the USA
This tPA Total ELISA (Enzyme-Linked Immunosorbent Assay) is for the quantitative analysis of tPA levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free, latent and complexed tPA is quantified with the use of an HRP labeled secondary antibody.
The various forms of tPA present in the standard or unknown is captured by the tPA capture antibody coated on the well. A primary antibody specific for tPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.