Cancer Biomarker ELISA Assay

Mouse Neprilysin ELISA Kit

$585.00

The Mouse Neprilysin ELISA Kit is intended for the quantitation of Mouse MME concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse CD10/Neprilysin ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: CD111-K01 Categories: , ,

Mouse Neprilysin ELISA Kit

The Mouse Neprilysin ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 93.7pg/ml-6000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: CD10, membrane metallo-endopeptidase, neutral endopeptidase, NEP, Common acute lymphoblastic leukemia antigen, CALLA

Assay Background

Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer’s disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Related Products

Human Neprilysin ELISA Kit
Mouse CD56/NCAM1 ELISA Kit
Mouse Cystatin C ELISA Kit

Additional Information

Assay Principle

The Mouse MME Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse MME with a 96-well strip plate that is pre-coated with antibody specific for MME. The detection antibody is a biotinylated antibody specific for MME. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse MME with immunogen: Expression system for standard: sf21; Immunogen sequence: Y52-W750. The kit is analytically validated with ready to use reagents. To measure Mouse MME, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse MME in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse MME in the sample.

Typical Standard Curve

Mouse Neprilysin ELISA Kit

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Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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