Mouse Monoclonal Anti-p53 Antibody Clone BP-53-12

Mouse Monoclonal Anti-p53 Antibody Clone BP-53-12

The Mouse Monoclonal Anti-p53 Antibody Clone BP-53-12 is For Research Use Only

Immunogen: N-terminal part of recombinant protein
Alternative Names: Tumor protein p53, cellular tumor antigen p53, the Guardian of the Genome, phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53
Host: Mouse
Class: IgG2a, kappa
Specificity: Human
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Colon adenocarcinoma
Demasking: Temperature, citrate buffer, pH 6 (12-310002)
Staining: Nuclear
Reagent provided: This antibody is purified immunoglobulin IgG2a diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
Usage dilution:
10-310009-7: Ready to Use
10-310009-01; 10-310009-05; 10-310009-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Citrate Buffer, pH 6.0 (REF 12-310002)
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.

Background: Antibodies bind to the normal and mutant types of p53 protein and are used to detect increased expression of p53. For many types of tumors, the detection of p53 is predictive. The wild-type p53 protein is present in normal cells, but, due to the extremely short half-life, it is not detected using standard immunohistochemical methods. Somatic mutations of the p53 gene are often found in various tumor processes. The mutant p53 protein is more stable than the wild-type protein and accumulates in tumor cells in significant concentration.

Product Developed and Manufactured by Diagomix.