Mouse Monoclonal Anti-ER Antibody Clone PBM-1H7
The Mouse Monoclonal Anti-ER Antibody Clone PBM-1H7 is For Research Use Only
Immunogen: Full size recombinant protein
Alternative Names: Anti-Estrogen Receptor
Host: Mouse
Class: IgG1, kappa
Specificity: Human
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: breast Cancer (ER +), uterine endometrium
Demasking: Temperature, citrate buffer, pH 6 (12-310002)
Staining: Nuclear
Reagent provided: This antibody is purified immunoglobulin IgG1, kappa diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
Usage dilution:
10-310035-7: Ready to Use
10-310035-01; 10-310035-05; 10-310035-1: Dilute 1:50 to 1:100 with antibody Diluent (12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Citrate Buffer, pH 6.0 (REF 12-310002)
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Background: Antibodies bind to cells that have estrogen-alpha (ER) receptors. Human estrogen receptors are responsible for the regulation of growth and differentiation of breast cells and have alpha and beta forms. In normal tissues, ERs are present in the epithelial cells of the mammary glands, uterine epithelium and myometrium, endometrium in the luteal phase. In breast cancer, most cells expressing progesterone receptors (PR) also express estrogen receptors, and only 5% of PR positive cells do not contain ER. Estrogen receptors are also expressed in some smooth muscle tumors, malignant melanomas, meningiomas, sclerosing hemangiomas, thyroid tumors, cervical cancer and ovarian cancer.
Product Developed and Manufactured by Diagomix.
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