Mouse IL-12 ELISA Assay


The Eagle Biosciences Mouse IL-12 ELISA Assay Kit is used as an analytical tool for quantitative determination of Mouse Interleukin 12, IL-12/P70 in serum and plasma. The Mouse IL-12 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Mouse IL-12 ELISA Assay

THe Mouse IL-12 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 4.52 ng/mL
Dynamic Range: 10 – 2000 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µl
Alternative Names: Interleukin 12

Assay Principle

The method employs sandwhich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 12, IL-12/P70 present in the sample are bound by the antibodies. Then, a HRP (horseradish peroxidase) conjugated secondary antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 12, IL-12/P70 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Assay Background

The Mouse IL-12/P70 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum / plasma as validated with the kit. The kit employs a sandwhich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibodies. Double antibodies are used in this kit.

Related Products

Mouse IL-12p70 ELISA Assay
Mouse IL-10 ELISA Assay

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations