Infliximab ELISA Assay Kit
The Infliximab ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Sensitivity: <2.5 ng/mL
Standard Range: 2.5-160 ng/ml
Incubation Time: 1.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants
Sample Size: 100 µL
Alternative Name: Remicade
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Infliximab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Infliximab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Infliximab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Infliximab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage:
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C. For Cell Culture Supernatant – If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at -20°C or -80⁰C. Avoid repeated freeze-thaw cycles.
Preparation Before Use: Allow samples to reach room temperature prior to assay. Take care to agitate patient samples gently in order to ensure homogeneity.
Test Sample preparation – Samples have to be diluted 1:10 to 1:100 (v/v), e.g. for 1:100 (5 µl sample + 495 µl sample diluent) prior to assay. The samples may be kept at 2 – 8°C for up to three days. Long-term storage requires -20°C.