iLite AAV Responsive Effector Cells
iLite AAV Responsive Effector Cells are Developed and Manufactured by Svar Life Science
The iLite AAV 8 Packaging Assay Ready Cells together with the iLite AAV Responsive Reporter Assay Ready Cells can be used as a transduction inhibition (TI) assay for the determination and titration of neutralizing antibodies against AAV vectors of serotype 8. Application Notes for the following assays are available:
- Determination of NAbs against AAV in human serum using iLite AAV Packaging and iLite AAV Responsive Reporter Assay Ready Cells
- Titration of NAbs against AAV in human serum using iLite AAV Packaging and iLite AAV Responsive Reporter Assay Ready Cells
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C or at lower temperature and are stable as supplied until the expiry date shown. Cells should be diluted and plated immediately after thawing.
For Research Use Only
Background
Currently, the AAV product development and approval processes have multiple challenges that require the development of new tools. The iLite® AAV Responsive Assay Ready Cells together with iLite® AAV Packaging Assay Ready Cells of any serotype represent a functional, robust, and agile technology to facilitate the determination and titration of neutralizing antibodies against AAV vectors.
Products Related to iLite Responsive Effector AAV Assay Ready Cells
iLite AAV 2 Packaging Assay Ready Cells
iLite AAV 5 Packaging Assay Ready Cells
iLite AAV 6 Packaging Assay Ready Cells
iLite AAV 8 Packaging Assay Ready Cells
iLite AAV 9 Packaging Assay Ready Cells
Additional Information
Summary
The iLite® AAV Responsive Reporter Assay Ready Cells are based on the human embryonic kidney 293 (HEK293) cell line and have been genetically engineered and optimized to be responsive to the transduction of AAV vectors produced by iLite®AAV Packaging Assay Ready Cells. The transduction leads to the activation of a transactivator regulating the Firefly Luciferasereporter gene construct.The cells also contain the Renilla Luciferase internal standardization gene under the control of a constitutive promoter that renders assay results independent of cell number and provides a means for correcting for cytotoxic effects that may be encountered with some biological samples. The Eagle Biosciences iLite® AAV Responsive Effector Assay Ready Cells are for research use only and not for use in diagnostic procedures.
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Package Inserts
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