iLite AAV Responsive Effector Cells

$1,690.00

The iLite® AAV Responsive Reporter Assay Ready Cells are based on the human embryonic kidney 293 (HEK293) cell line and have been genetically engineered and optimized to be responsive to the transduction of AAV vectors produced by iLite®AAV Packaging Assay Ready Cells. The transduction leads to the activation of a transactivator regulating the Firefly Luciferasereporter gene construct.The cells also contain the Renilla Luciferase internal standardization gene under the control of a constitutive promoter that renders assay results independent of cell number and provides a means for correcting for cytotoxic effects that may be encountered with some biological samples. The Eagle Biosciences iLite® AAV Responsive Effector Assay Ready Cells are for research use only and not for use in diagnostic procedures.

iLite AAV Responsive Effector Cells

iLite AAV Responsive Effector Cells are Developed and Manufactured by Svar Life Science

The iLite AAV 8 Packaging Assay Ready Cells together with the iLite AAV Responsive Reporter Assay Ready Cells can be used as a transduction inhibition (TI) assay for the determination and titration of neutralizing antibodies against AAV vectors of serotype 8. Application Notes for the following assays are available:

  • Determination of NAbs against AAV in human serum using iLite AAV Packaging and iLite AAV Responsive Reporter Assay Ready Cells
  • Titration of NAbs against AAV in human serum using iLite AAV Packaging and iLite AAV Responsive Reporter Assay Ready Cells

Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C or at lower temperature and are stable as supplied until the expiry date shown. Cells should be diluted and plated immediately after thawing.

For Research Use Only



Background

Currently, the AAV product development and approval processes have multiple challenges that require the development of new tools. The iLite® AAV Responsive Assay Ready Cells together with iLite® AAV Packaging Assay Ready Cells of any serotype represent a functional, robust, and agile technology to facilitate the determination and titration of neutralizing antibodies against AAV vectors.


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Additional Information

Summary


The iLite® AAV Responsive Reporter Assay Ready Cells are based on the human embryonic kidney 293 (HEK293) cell line and have been genetically engineered and optimized to be responsive to the transduction of AAV vectors produced by iLite®AAV Packaging Assay Ready Cells. The transduction leads to the activation of a transactivator regulating the Firefly Luciferasereporter gene construct.The cells also contain the Renilla Luciferase internal standardization gene under the control of a constitutive promoter that renders assay results independent of cell number and provides a means for correcting for cytotoxic effects that may be encountered with some biological samples. The Eagle Biosciences iLite® AAV Responsive Effector Assay Ready Cells are for research use only and not for use in diagnostic procedures.

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