Human Soluble alpha-Klotho ELISA
Human Soluble alpha-Klotho ELISA Developed and Manufactured by Immuno-Biological Laboratories Co., Ltd
Size: 1×96 wells
Sensitivity: 6.15 pg/mL
Standard Range: 0 / 93.75 – 6000 pg/mL
Incubation Time: 2 hours
Sample Type: Serum, plasma (EDTA, Heparin, Citrate), urine and cell culture supernatant
Sample Size: 100 µl
Assay Background
α-Klotho is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis. α-klotho was identified as an extremely down-regulated gene in the genetic mutation mouse (Klotho mouse) of which phenotype is very similar to various symptoms of human aging. Then, the sequences of α-Klotho genes have been identified in various species including human based on the one of mouse. α-Klotho protein is a 130kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α- Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of α-Klotho protein is released free into blood by shedding. However, there are may unclear points about functions and changes in concentration of free (soluble) α-Klotho protein, so it has been required to develop the measurement system of α-Klotho. Human soluble α-Klotho protein in human blood and urine can be measured by this assay kit. Solid phase sandwich ELISA for the determination of human soluble Alpha Klotho ( α-Klotho ) in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures.
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