Cancer Biomarker ELISA Assay

Human Neprilysin ELISA Kit

$585.00

The Eagle Biosciences Human CD10/Neprilysin ELISA Kit is intended for the quantitation of Human MME concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human CD10/Neprilysin ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: CD131-K01 Categories: , ,

Human Neprilysin ELISA Kit

The Human Neprilysin ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 125pg/ml-8000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: CD10, membrane metallo-endopeptidase, neutral endopeptidase, NEP, Neprilysin, or common acute lymphoblastic leukemia antigen, CALLA

Assay Background

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer’s disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Related Products

Mouse CD10/Neprilysin ELISA Kit
Human LIFR ELISA Kit
Human Livin ELISA Kit

Additional Information

Assay Principle

The Human MME Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MME with a 96-well strip plate that is pre-coated with antibody specific for MME. The detection antibody is a biotinylated antibody specific for MME. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MME with immunogen: Expression system for standard: CHO; Immunogen sequence: Y52-W750. The kit is analytically validated with ready to use reagents. To measure Human MME, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MME in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MME in the sample.

Typical Standard Curve

Documents

Product Documents

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations