Human KIM-1 ELISA Assay

$580.00

The Human KIM-1 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological samples of Humans.

SKU: HKM39-K01 Categories: , , Tags: ,

Human KIM-1 ELISA Assay

The Human KIM-1 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.054 ng/ml
Dynamic Range: 0.125 ng/ml – 4 ng/ml
Incubation Time: 110 minutes
Sample Type: Plasma, Serum, Urine
Sample Size: 100 μL
Alternative Names: Kidney injury molecule 1, KIM1


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti- KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. For urine samples, collect using standard techniques. The samples should be centrifuged to remove any possible debris. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.


Related Products

Canine KIM-1 ELISA Assay
Mouse KIM-1 ELISA Assay Kit
Rat KIM-1 ELISA Assay

Additional Information

Assay Background


KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.

Typical Standard Curve


Manual

Product Manual