Human Insulin Biotinylated

$245.00

The Human Insulin Biotinylated (Biotin-Insulin) is for research use only and not for use in diagnostic procedures.

SKU: INS30-G100 Category:

Human Insulin Biotinylated

The Human Insulin Biotinylated is For Research Use Only
Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH

Aliquot: 100 µg
Storage: 4°C for up to 1 year
Alternative Names: Biotin-Insulin


Product Specifications

  • Quantity: 100 µg
  • Description: Recombinant human Insulin produced in yeast has been biotinylated using our biotinylation technology that was developed for IGF`s and analogs.
  • Formulation: Lyophilized from a solution of 0.5 ml TBS, pH 7.4, containing 100 µg (0.1 mg) of the biotinylated product.  A preservative and a stabilizing protein of non-mammalian origin are added to ensure stability of the reconstituted product.
  • Storage: Store refrigerated upon arrival. The product is stable at 4 °C for at least one year.  Reconstitute with 0.5 ml water and store aliquots frozen at – 20 °C. Avoid repeated freeze-thawing cycles.

Advantages of Our Exceptional Biotinylation Technology:
This biotinylation technology has been designed and developed for Insulin and IGF´s that is different from the published methods.  Our technology permits biotinylation without loss of activity.

Our Biotinylated Insulin and IGF´s and analogs bind to:

  • Native IGFBP´s and IGFBP´s on western blots
  • Antibodies

Our Biotinylated Insulin and IGF´s also:

  • Bind to receptors, they are internalized, and transported through cell layers
  • Biologically active in-vitro and in-vivo

Related Products

Biotinylated Human Des IGF-1
IGF-1 Biotinylated (Biotin-(Leu24) IGF-1)
Biotinylated Human IGF-2

Additional Information

Why has insulin a continuously emerging field of research?


Diabetes truly begins and ends on a microscopic level in the pancreas within a cluster of islets containing beta cells called the Islets of Langerhans. This is where millions of islet cells coordinate intermittent secretory bursts of insulin into the portal veins. Beta cells normally release insulin so that the glucose can be efficiently metabolized in normal, healthy people. However, in diabetic individuals, beta cells fail, resulting in their inability to appropriately respond to increases in glucose in the blood. Glucose is the principal stimulus for insulin secretion, though other macronutrients (ie. Carbohydrates, proteins, fats), hormones, humoral factors and neural input may modify this response.1,2
Insulin and insulin resistance has been at the forefront of medical research with obesity and diabetes reaching epidemic levels across the world. There are various fields of research that surround measuring, analyzing, and understanding this biomarker and its role across a wide range of physiological processes. The influences on its synthesis and secretion, in addition to its actions from the cellular level to the whole body level, has significant implications for much chronic disease.1 It is only in scientific laboratories that insulin’s complex actions at the cellular level can be observed. 1

Why would you want to use Recombinant Biotinylated Human Insulin?


As described above, insulin has quite an intricate role in the body and a significant impact on various organs, tissues, and metabolic functions which makes uniform testing impossible for researchers. Laboratories worldwide have dedicated their time and resources to various aspects of diabetes and obesity research ranging from general insulin and insulin resistance studies to islet cell replacement studies. Our Recombinant Biotinylated Human Insulin is a stable, multi-method tool for every diabetes and obesity research need.

Manual

Product Manual


Publications

References


  1. Wilcox, Gisela. Insulin and Insulin Resistance. Clin Biochem Rev. 2005 May; 26(2): 19–39.
  2. German, Michael et al.   https://www.diabetes.org/assets/pdfs/forefront/forefront-15-summerfall-2011.pdf

Citations

Stensland, ZC., Smith, MJ. Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry. Magnetochemistry (2021)7; 7:92

Wan, X;Thomas, JW;Unanue, ER; Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites; J. Exp. Med., pg.967-78, 213:6,2016,doi: 10.1084/jem.20151869.

Zhai, N., Liu, C., Van Ingelgom, A., Martens, Y., Knight, C., Painter, M., . . . Bu, G. (2017, September 27). Apolipoprotein E4 Impairs Neuronal Insulin Signaling by Trapping Insulin Receptor in the Endosomes [Abstract]. Neuron, 96(1), 115-129.e5. doi:10.1016/j.neuron.2017.09.003

Product Citations