Biotinylated IGFBP-2 Recombinant Protein

$375.00

SKU: BP230-G2.5 Category:

Biotinylated IGFBP-2 Recombinant Protein

The Biotinylated IGFBP-2 Recombinant Protein is For Research Use Only

Product Specifications


  • Biotinylated Human IGFBP-2 has been prepared from receptor grade recombinant human IGFBP-2.The product was purified using chromatographic techniques.
  • Appearance: Lyophilized with no additives.
  • Reconstitution: Reconstitute 2.5 µg aliquot with 0.1 ml double distilled water or buffer of your choice.
  • Storage: The product is shipped at room temperature, for long term storage store at – 20 °C. Reconstituted samples can be stored for at least one week at 2-8 °C.

Application Notes
Biotinylated IGF´s and analogs have found a wide range of applications in in-vitro and in-vivo and are a safe and stable alternative to 125 I-lableld IGF´s. As 125 I-lableld IGFBP´s have also been used for methods as e.g. proteolysis assays, binding studies and in-vivo studies, there should be some potential for biotinylated IGFBP´s, too. A literature search on the use of biotinylated IGFBP´s resulted in a small number of reports, which are limited to IGFBP-1(1-3), IGFBP-2 (4,5), IGFBP-3 (6-14, 16) and IGFBP-4 (15). Until today we found no scientific papers on the use of biotinylated IGFBP-5 and IGFBP-6.
Though there is small number of papers, the authors have used the biotinylated IGFBP´s in a broad range of techniques to study IGF-dependent and IGF-independent actions of IGFBP´s. Biotinylated IGFBP´s have been used in immunoassays (1, 6, 11, 15), proteolysis studies (2,3), ligand blot (4,10, 12, 16), cross-linking studies (9, 12) and binding studies (1, 4, 5, 7, 8, 13). Biotinylated IGFBP-3 has been used to demonstrate the nuclear appearance of IGFBP-3 invivo (14).


Related Products

Human IGFBP-3 Recombinant Protein, Biotin-labeled
Human IGFBP-4 Recombinant Protein, Biotin-labeled
Human IGFBP-5 Recombinant Protein, Biotin-labeled
Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH

Manual

Product Manual


Publications

References


  1. Pereira et al.: Bimolecular Interaction of Insulin-Like Growth Factor (IGF) Binding Protein-2 with  anb3 Negatively Modulates IGF-I-Mediated Migration and Tumor Growth. CANCER RESEARCH 64, 977–984, February 1, 2004.
  2. Ben et al.: DIFFERENTIATIVE EFFECTS OF IGF SYSTEM COMPONENTS ON BOVINE FOLLICULAR CELLS. Biology of Reproduction, Volume 64, Supplement 1.  Society for the Study of Reproduction, 34th Annual Meeting, July 28 – August 1, 2001, University of Ottawa.