Biotinylated IGFBP-2 Recombinant Protein

$415.00

SKU: BP230-G2.5 Category:

Biotinylated IGFBP-2 Recombinant Protein

The Biotinylated IGFBP-2 Recombinant Protein is For Research Use Only

Product Specifications


  • Biotinylated Human IGFBP-2 has been prepared from receptor grade recombinant human IGFBP-2.The product was purified using chromatographic techniques.
  • Appearance: Lyophilized with no additives.
  • Reconstitution: Reconstitute 2.5 µg aliquot with 0.1 ml double distilled water or buffer of your choice.
  • Storage: The product is shipped at room temperature, for long term storage store at – 20 °C. Reconstituted samples can be stored for at least one week at 2-8 °C.

Application Notes
Biotinylated IGF´s and analogs have found a wide range of applications in in-vitro and in-vivo and are a safe and stable alternative to 125 I-lableld IGF´s. As 125 I-lableld IGFBP´s have also been used for methods as e.g. proteolysis assays, binding studies and in-vivo studies, there should be some potential for biotinylated IGFBP´s, too. A literature search on the use of biotinylated IGFBP´s resulted in a small number of reports, which are limited to IGFBP-1(1-3), IGFBP-2 (4,5), IGFBP-3 (6-14, 16) and IGFBP-4 (15). Until today we found no scientific papers on the use of biotinylated IGFBP-5 and IGFBP-6.
Though there is small number of papers, the authors have used the biotinylated IGFBP´s in a broad range of techniques to study IGF-dependent and IGF-independent actions of IGFBP´s. Biotinylated IGFBP´s have been used in immunoassays (1, 6, 11, 15), proteolysis studies (2,3), ligand blot (4,10, 12, 16), cross-linking studies (9, 12) and binding studies (1, 4, 5, 7, 8, 13). Biotinylated IGFBP-3 has been used to demonstrate the nuclear appearance of IGFBP-3 invivo (14).


Related Products

Human IGFBP-3 Recombinant Protein, Biotin-labeled
Human IGFBP-4 Recombinant Protein, Biotin-labeled
Human IGFBP-5 Recombinant Protein, Biotin-labeled
Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH

Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Publications

References


  1. Pereira et al.: Bimolecular Interaction of Insulin-Like Growth Factor (IGF) Binding Protein-2 with  anb3 Negatively Modulates IGF-I-Mediated Migration and Tumor Growth. CANCER RESEARCH 64, 977–984, February 1, 2004.
  2. Ben et al.: DIFFERENTIATIVE EFFECTS OF IGF SYSTEM COMPONENTS ON BOVINE FOLLICULAR CELLS. Biology of Reproduction, Volume 64, Supplement 1.  Society for the Study of Reproduction, 34th Annual Meeting, July 28 – August 1, 2001, University of Ottawa.

Product Citations