The Human Complement H/CFH ELISA Kit is intended for the quantitation of Human CFH concentrations in cell culture supernates, serum, plasma (heparin, EDTA) and urine. The Eagle Biosciences Human Complement H/CFH ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: HCH31-K01 Categories: , ,


The Human CFH ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <50pg/ml
Standard Range: 1.56ng/ml-100ng/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Serum, Plasma (heparin, EDTA) and Urine.
Sample Size: 100 µl
Alternative Name: Complement Factor H, beta-1H globulin

Assay Principle

The Human CFH Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CFH with a 96-well strip plate that is pre-coated with antibody specific for CFH. The detection antibody is a biotinylated antibody specific for CFH. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CFH with immunogen: Expression system for standard: NSO, Immunogen sequence: S860-R1231. The kit is analytically validated with ready to use reagents. To measure Human CFH, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CFH in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human CFH in the sample.

Related Products

Human complement C5a ELISA Kit
Complement TCC ELISA Kit
Mouse Complement H/CFH ELISA Kit

Additional Information

Assay Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for ‘regulators of complement activation’). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Typical Standard Curve



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