Eagle Bio Antibodies

Human Artemin Mouse Monoclonal Antibody Clone 2D12

$610.00

Recombinant human Artemin protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant.

SKU: 324-100 Categories: ,

Human Artemin Mouse Monoclonal Antibody Clone 2D12

The Human Artemin Mouse Monoclonal Antibody Clone 2D12 is For Research Use Only

Immunogen: Human Artemin
Uniprot ID: Q5T4W7
Alternative Names: Enovin, Neublastin, ARTN
Clonality: Mouse monoclonal
Clone:: 2D12
Class: IgG1
Reactivity: Human Artemin
Application: ELISA, WB, IF
Purification: IgG fraction purified with Protein G affinity chromatography
Buffer: PBS pH 7.4, with 0.1% sodium azide
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Monoclonal and polyclonal antibodies and ELISA kit to human Artemin.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at –20 to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Specifications

Protocol:
ELISA 1:500 to 1:5 000; Western immunoblotting 1:500 – 1:2000 (detects only dimeric protein in non-reducing conditions); IF 1:50 – 1:3000. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Background:
Artemin supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer’s patch-like structures, a major component of the gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex (UniProt).

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Product Developed and Manufactured by Icosagen

Additional Information

Figure 1:
Human Artemin Mouse Monoclonal Antibody Clone 2D12

Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2D12. Goat anti-mouse IgG HRP conjugate was used as secondary antibody. 15 ml of  CHOEBNALT85 Artemin expressing supernatant was loaded per line. Line 1. Reduced conditions; Line 2. Non-reduced conditions; Line 3. Protein marker (8003, Naxo)AB

Figure 2:
Human Artemin Mouse Monoclonal Antibody Clone 2D12
Human Artemin Mouse Monoclonal Antibody Clone 2D12

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin.  Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2D12. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control – GFRa3-Ret51 receptor expressing U2OS cells.

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