Human Apolipoprotein H (ApoH) ELISA Assay Kit

$480.00

The Eagle Biosciences Human Apolipoprotein H (ApoH) ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Apo-H in biological fluids of Human.

Human Apolipoprotein H (ApoH) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Standard Range: 3.125 – 200 ng/ml
Incubation Time: 90 minutes
Sample Type: Biological Fluids
Sample Size: 100 µl

Product manufactured in the USA

Additional Information

Assay Principle


The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Apo-H present in samples reacts with the anti-Apo-H antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Apo-H antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Apo-H. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Apo-H in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Apo-H in the test sample. The quantity of Apo-H in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Typical Standard Curve


Manual

Product Manual