HSP65 ELISA Assay Kit

$690.00$3,090.00

The HSP65 ELISA Assay Kit is for the detection of Hsp65 in cell lysates, tissue extracts, and serum samples.

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SKU: SKT-113 Categories: , ,

HSP65 ELISA Assay Kit

The HSP65 ELISA Assay Kit is For Research Use Only

Sensitivity: 0.06 ng/ml
Dynamic Range: 0.344 – 22 ng/ml
Incubation Time: 30 minutes
Sample Type: Cell lysates, Serum, Tissue
Sample Size: 100 μL
Assay Specificity and Species Reactivity
• This assay is specific for Hsp65
• The Hsp65 ELISA has been validated for the detection of Hsp65

Product manufactured in Canada by StressMarq.


Assay Background

HSP65 is a member of the HSP60 family of heat shock proteins. HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner. Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively. Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats).


Storage and Stability
All reagents are stable as supplied at 4°C. Unused wells should be resealed with desiccant in the foil pouch provided, and stored at 4°C until the kits expiry date.

Related Products

HSP60 ELISA Assay Kit
HSP27 ELISA Assay Kit
HSP70 ELISA Assay Kit

Additional Information

Assay Principle


  1. Prepare Standard and samples in Standard and Sample Diluent.
  2. Add 100 µL of Standard or sample to appropriate wells.
  3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour.
  4. Wash plate four times with 1X Wash Buffer.
  5. Add 100 µL of Biotinylated Antibody Working Solution to each well.
  6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
  7. Wash plate four times with 1X Wash Buffer.
  8. Add 100 µL of Streptavidin-HRP Working Solution to each well.
  9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
  10. Wash plate four times with 1X Wash Buffer.
  11. Add 100 µL of TMB Substrate to each well.
  12. Develop the plate in the dark at room temperature for 30 minutes.
  13. Stop reaction by adding 100 µL of Stop Solution to each well.
  14. Measure absorbance on a plate reader at 450 nm.

Typical Standard Curve


HSP65 ELISA Assay Kit

Manual

Product Manual