High Sensitivity Glucagon ELISA
The High Sensitivity Glucagon ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.3 pmol/L
Dynamic Range: 2.2-143.6 pmol/L
Incubation Time: 18-20 hours
Sample Type: Plasma, Serum and Culture Medium Supernatant
Sample Size: 10 μL
Alternative Name: HS Glucagon ELISA
Stability and storage
Store all of the components at 2-8ºC.
The kit is stable under the condition for 6 months
from the date of manufacturing.
The expiry date is stated on the label of kit.
Glucagon is a 29-amino acid polypeptide hormone, synthesized and secreted from alpha cells of the islets of Langerhans. Glucagon generally elevates the concentration of glucose in the blood by promoting gluconeogenesis and glycogenolysis, and it has the opposite effect of insulin. The secretion of glucagon and insulin into the blood in response to the blood glucose concentration is the primary mechanism responsible for keeping the glucose levels. Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit is a sandwich ELISA that has been developed by using two monoclonal antibodies specific for glucagon. This kit has a high specificity to glucagon and shows no significant cross reactivity with Glicentin, Oxyntomodulin, GLP-1 and GLP-2.
Glucagon ELISA Assay Kit
Human GLP-2 ELISA Assay Kit
GLP-1 Total ELISA Assay
This ELISA kit for determination of glucagon is based on a sandwich enzyme immunoassay with two monoclonal antibodies. Standards or samples, and HRP labeled antibodies are added to the wells of plate coated with antibodies against glucagon. During the incubation antibody – antigen – labeled antibody complex is formed on the surface of the wells. After the incubation and rinsing out excess labeled antibody, HRP enzyme activity is finally determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of glucagon is calculated.
- Before starting the assay, bring all the reagents and samples to room temperature (20 ~ 30ºC).
- Fill 0.35 mL/well of washing solution into the wells and aspirate the washing solution in the wells. Repeat this washing procedure further twice (total 3 times). Finally, invert the plate and tap it onto an absorbent surface, such as paper toweling, to ensure blotting free of most residual washing solution.
- Add 10µL of each of standard solutions (0, 2.2, 4.5, 9.0, 17.9, 35.9, 71.8 and 143.6 pmol/L) or samples to the wells first, and then 100 µL of HRP labeled antibody solution to each of the wells.
- Cover the plate with adhesive foil and incubate it at 2 ~ 8ºC for 18 ~ 20 hours (keep still, plate shaker not need).
- After incubation, take off the adhesive foil, aspirate and wash the wells 6 times with 0.35 mL/well of washing solution. Finally, invert the plate and tap it onto an absorbent surface, such as paper toweling, to ensure blotting free of most residual washing solution.
- Add 100µL of Enzyme substrate solution (TMB) to each of the well, cover the plate with adhesive foil and keep it for 30 minutes at room temperature in a dark place for color reaction (keep still, plate shaker not need).
- Add 100 µL of stopping solution to each of the wells to stop color reaction.
- Read the optical absorbance of the solution in the wells at 450 nm. The dose-response curve of this assay fits best to a 5 (or 4)-parameter logistic equation. The results of unknown samples can be calculated with any computer program having a 5 (or 4)-parameter logistic function.
This Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit has high specificity to glucagon, and shows no significant cross reactivity to Glicentin, Oxyntomodulin, GLP-1 and GLP-2.