High Sensitive Histamine ELISA Assay Kit
For Research Use Only
Size: 1×96 wells
Sensitivity: 0.2 ng/ml
Standard Range: 0.5 – 50 ng/ml
Incubation Time: overnight, 2 x 30 min
Sample Type: various biological samples
Sample Size: 10 µl
Controls Included
Assay Principle
During the sample preparation Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
