GST Pi Assay Kit
The GST Pi Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.28 ng/mL
Standard Range: 0.25-10 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Urine
Sample Size: 100 µl
Assay Background
The human cytosolic Glutathione S-Transferases (GSTs) are expressed as 18 distinct gene products yet they share a common structural morphology. This immunoassay is specific for GST Pi (GSTP) and exhibits less than 1% cross reactivity towards the other 17 human cytosolic GST enzymes. GSTP is considered to be a urinary biomarker for renal cell damage and is localized to the distal convoluted tubules, thin Loop of Henle and the collecting ducts of the kidney.
SAMPLE STORAGE
Samples should be stored at –80°C and thawed just prior to use. Avoid repeated freeze/thaw cycles for best results. This assay was developed and validated with human serum and urine samples, however it can also be used for plasma samples. NOTE: the use of serum samples for assessing the level of circulating GSTP in blood will give artificially higher levels due to the release of GSTP from platelets during the clotting process.
SAMPLE PREPARATION
It is recommended to do multiple sample dilutions to ensure that the concentration falls within the accepted range for the assay. Urine samples should be diluted at least 1:2 in Assay Buffer for best results. Serum and Plasma samples should not be run neat and are recommended to be run diluted at least 1:3 in Assay Buffer.
