Golimumab (SIMPONI) ELISA Assay Kit
The Golimumab (SIMPONI) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.01 ug/mL
Standard Range: 0 – 3 ug/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Name: Simponi
Assay Background
Golimumab is a human IgGl K monoclonal antibody derived from immunizing genetically engineered mice with human TNFa. Golimumab binds and inhibits soluble and transmembrane human TNFa. As a human monoclonal antibody, golimumab binds and inhibits soluble and transmembrane human TNFa. Inhibition of TNFa prevents it binding to its receptors, which prevents both leukocyte infiltration through prevention of cell adhesion proteins such as E-selectin, ICAM-1 and VCAM-1, and pro-inflammatory cytokine secretion such as IL-6, IL-8, G-CSF and GM-CSF in vitro. Consequently, in patients with chronic inflammatory conditions, decreases in ICAM-1 and IL-6 as well as C-reactive protein (CRP), matrix metalloproteinase 3 (MMP-3), and vascular endothelial growth factor (VEGF) were observed.
Assay Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for golimumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to golimumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of golimumab in the sample or standard. Results of samples can be determined directly using the standard curve.
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