GLP-1 Total ELISA Assay Kit

$580.00

The Eagle Biosciences GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) (GLP-1 (7-36) and GLP-1 (9-36)) in plasma samples.  Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: GP131-K01 Categories: , ,

GLP-1 Total ELISA Assay Kit

For Research Use Only

Size:  1×96 wells
Sensitivity:  0.6 pmol/L
Dynamic Range:  2 -54 pmol/L
Incubation Time:  overnight
Sample Type:  Plasma  
Sample Size: 100 µL

Controls Included

Product Developed and Manufactured in the USA


Additional Information

Assay Principle

This GLP-1 Total ELISA Assay Kit is designed, developed and produced for the quantitative measurement of GLP-1 (7-36) and (9-36) in plasma sample. The GLP-1 Total ELISA Assay Kit utilizes the two-site “sandwich” technique with two selected GLP-1 antibodies.

Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36)/(9-36) – HRP-conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

Assay Procedure

  1. Place a sufficient number of streptavidin-coated microwell strips/wells (Cat. 10040B) in a holder to run Total GLP-1 standards, controls and unknown samples in duplicate.
  2. Add 100 µL of standards, controls and test samples into the designated microwell.
  3. Add 100 µL of Total GLP-1 Antibody Mixture to each well
  4. Cover the plate with one plate sealer and incubate plate at 2-8°C, static for 20 – 24 hours.
  5. Remove plate sealer. Aspirate the contents of each well. Wash each well 5 times by dispensing 350 µL of working wash solution into each well and then completely aspirating the contents. Alternatively, an automated microplate washer can be used.
  6. Add 200 µL of ELISA HRP Substrate into each of the wells.
  7. Cover the plate with one plate sealer and also with aluminum foil to avoid exposure to light.
  8. Incubate plate at room temperature, static for 20 min.
  9. Remove the aluminum foil and plate sealer. Add 50 µL of ELISA Stop Solution into each of the wells. Mix gently
  10. Read the absorbance at wavelength 450nm/620 nm within 10 minutes in a microplate reader.

Typical Standard Curve