Glicentin ELISA Assay Kit
The Glicentin ELISA Assay Kit was manufactured in Sweden by Mercodia
Size: 12 x 8 wells
Sensitivity: 3 pmol/L (24 pg/mL)
Dynamic Range: 3 – 300 pmol/L (24-2400 pg/mL)
Incubation Time: 2 hours 15 minutes
Sample Type: Serum, EDTA plasma, cell-culture medium
Sample Size: 25 µL
Assay Background
Glicentin, a polypeptide composed of 69 amino acids, is derived from the posttranslational cleavage of proglucagon in the gut. The sequence of glicentin also include the sequences of glucagon, mini-glucagon, oxyntomodulin and GRPP, all of which are derived from proglucagon. Observed biological actions of glicentin are stimulation of insulin secretion, inhibition of gastric acid secretion, control of gut motility, and stimulation of gut growth. These properties are also attributed to GLP-1 and GLP-2, making it unclear if the high concentrations of glicentin used in some studies resulted in minor activation of GLP-1 and GLP-2 receptors.
Assay Principle
The Glicentin ELISA Assay Kit is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the glicentin molecule. During incubation glicentin in the sample reacts with anti-human glicentin antibodies bound to microtitration well (clone C7H9G). After washing, which removes non-reactive plasma components, a peroxidase conjugated anti-glicentin antibody (clone RG-OC5) recognizes the glicentin bound to the solid phase. After a second incubation and a simple washing step that removes unbound enzyme labeled antibody, the bound conjugate is detected by reaction with 3,3’, 5,5’-tetramethylbenzidine (TMB). The reaction is stopped by adding acid to give a colorimetric endpoint, then read spectrophotometrically.
