GFAP CLIA Assay Kit
The GFAP CLIA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: <10 pg/ml
Dynamic Range: 10 – 640 pg/mL
Incubation Time: 2.5 hours
Sample Type: Serum, CSF
Sample Size: 100 µL
Key Benefits of this test from Eagle Biosciences
- Easy to run assay
- Run both Serum and CSF samples
- The MOST SENSITIVE Assay kit available in the world
Assay Principle
The GFAP CLIA test is based on the principle of a solid phase enzyme-linked sandwich immunosorbent assay. The assay system utilizes a specific monoclonal antibody directed against a distinct antigenic determinant on the GFAP molecule and is coated on the microtiter wells for the solid phase immobilization of GFAP. A biotin labeled rabbit anti-GFAP antibody is used as a reporter molecule in a sandwich immunoassay and streptavidin conjugated to Horse radish peroxidase (HRP) is used as the detector molecule. The test sample (serum) is allowed to react with the capture antibody which immobilizes GFAP present in the sample. Following washing, biotinylated polyclonal reporter antibody is added to the wells resulting in the GFAP molecule being sandwiched between the solid phase and biotin-labeled antibodies. After an additional incubation with streptavidin-HRP the wells are washed, a chemiluminescent substrate solution is added and the relative luminescence units (RLU) are measured spectrophotometrically using a luminometer. The concentration of GFAP is directly proportional to the RLUs of the test sample and is determined from the standard curve.
SPECIMEN COLLECTION AND PREPARATION
The use of serum samples is required for this test. Serum specimens from patients should be collected using standard techniques. Specimens which cannot be assayed within 6 hours after collection may be frozen at -20° C or lower and will be stable for up to six months. Specimens should not be repeatedly frozen and thawed prior to testing. DO NOT store in “frost free” freezers, which may cause occasional thawing. Specimens which have been frozen, and those which are turbid and/or contain particulate matter, must be centrifuged prior to use.
