CXCL 12 / SDF-1 Alpha ELISA Assay Kit

$790.00

The Eagle Biosciences CXCL 12 / SDF-1 Alpha ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of CXCL 12 / SDF-1 Alpha in serum and plasma. The CXCL 12 / SDF-1 Alpha ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

SKU: CXC31-K01 Categories: , ,

CXCL 12 / SDF-1 Alpha ELISA Assay Kit

For Research Use Only (RUO)

Size: 1×96 wells
Sensitivity: 0.31 ng/ml
Dynamic Range: 0.625 – 40 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 50 µL

Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH

Additional Information

Assay Principle

The biotinylated antibody of the CXCL 12 / SDF-1 Alpha ELISA Assay kit binds to the streptavidin coated microtiterplate. The SDF-1 α of the standards/samples binds to this antibody and is detected by the primary antibody. The biotinylated antibody, the primary antibody and the standards/samples are incubated on the microtiterplate altogether at the same time. The primary antibody, which is bound to SDF-1 Alpha, is then detected by a secondary peroxidase-conjugated antibody. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of SDF-1 Alpha.


Typical Assay Curve

Comparison of Serum vs. Plasma Samples

Manual

Product Manual