Complement Factor H-Related Protein 5 (FHR-5) ELISA Assay Kit


The Complement Factor H-Related Protein 5 (FHR-5) ELISA Assay Kit is to be used for the in vitro quantitative determination of FHR-5 in serum and plasma samples. The Complement Factor H-Related Protein 5 (FHR-5) is for research use only and not to be used for diagnostic procedures.

Complement Factor H-Related Protein 5 (FHR-5) ELISA Assay Kit

The Complement Factor H-Related Protein 5 (FHR-5) ELISA Assay Kit is For Research Use Only

Sizes: 1 x 96 wells; 2 x 96 wells
Sensitivity: 0.16 ng/mL
Dynamic Range: 0.16 – 10 ng/mL
Sample Size: 10 uL
Incubation Time: 1 hour 15 minutes
Sample Type: Serum and Plasma

Controls Not Included

Assay Background

Complement Factor H-related protein 5 (FHR-5) is a vital glycoprotein in the complement regulatory protein family. Predominantly synthesized in the liver, FHR-5, Human, also sees production from monocytes, macrophages, and dendritic cells. Weighing approximately 65 kDa, it structurally resembles Complement Factor H (CFH), a crucial regulator in the complement system. FHR-5’s primary role lies within the alternative pathway (AP) of the complement system. FHR-5’s functionality extends to binding with various host ligands, including C3b, heparin, C-reactive protein (CRP), Pentraxin 3 (PTX3), and elements of the extracellular matrix. It actively promotes alternative pathway activation on cell surfaces, offering a scaffold for AP C3 convertase assembly. Moreover, FHR-5 may challenge Factor H (FH) by competing for ligand binding, potentially influencing FH’s regulatory activities. Disease associations of FHR-5 are significant yet complex. Genetic variations in the CFHR5 gene have been identified in patients with atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Furthermore, its presence in both circulating and glomerular forms is linked to IgA nephropathy (IgAN) and familial C3 glomerulopathy (C3G). However, the specific role of FHR-5 in these conditions remains under debate, and other genetic factors may also contribute, underscoring the necessity for further research in this area. FHR-5, Human, can be measured with our HK3007 assay, designed to precisely quantify this intriguing glycoprotein.

Assay Principle

The Complement Factor H-Related Protein 5 (FHR-5) ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human FHR-5. Peroxidase-conjugated antibody will bind to the captured FHR-5. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human FHR-5 standards (log). The human FHR-5 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Related Products

Complement Factor H-Related Protein 2 (FHR-2) ELISA Assay Kit
Complement Factor H-Related Protein 3 (FHR-3) ELISA Assay Kit
Complement Factor H-Related Protein 4 (FHR-4) ELISA Assay Kit


Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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