Complement Factor H-Related Protein 2 (FHR-2) ELISA Assay Kit


The Complement Factor H-Related Protein 2 ELISA Assay Kit is to be used for the in vitro quantitative determination of FHR-2 in serum and plasma samples. The Complement Factor H-Related Protein 2 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Complement Factor H-Related Protein 2 (FHR-2) ELISA Assay Kit

The Complement Factor H-Related Protein 2 (FHR-2) ELISA Assay Kit is For Research Use Only

Sizes: 1 x 96 wells; 2 x 96 wells
Sensitivity: 0.88 ng/mL
Dynamic Range: 0.88 – 10 ng/mL
Sample Size: 10 uL
Incubation Time: 1 hour 15 minutes
Sample Type: Serum and Plasma

Controls Not Included

Assay Background

Complement Factor H-Related Protein 2 (FHR-2) is a significant member of the complement regulatory protein family, with a molecular weight of around 29 kDa. Synthesized mainly in the liver, FHR-2 is also produced by immune cells like monocytes, macrophages, and dendritic cells. Sharing structural homology with Complement Factor H (CFH), FHR-2 plays a role in regulating the complement cascade, although its exact biological function remains somewhat elusive and subject to debate. Research indicates that FHR-2 may inhibit the activity of the alternative pathway (AP) C3 convertase, which would theoretically prevent the formation of the membrane attack complex (MAC). Conversely, some studies suggest that FHR-2 might actually promote AP activation, highlighted by findings where a hybrid mutant protein comprising FHR-5 domains and full-length FHR-2 led to overactivation of the complement system. FHR-2 has been linked to various diseases where abnormal complement activation is a contributing factor. Particularly, it is associated with atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD), where dysregulation in complement activation leads to detrimental tissue deposition. The involvement of FHR-2 in these pathologies, specifically in the alternative pathway, makes it a potential therapeutic target in managing diseases driven by complement dysregulation. FHR-2 can be measured with our HK3004 assay, designed to precisely quantify this intriguing glycoprotein.

Assay Principle

The human FHR-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human FHR-2. Peroxidase-conjugated antibody will bind to the captured FHR-2. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human FHR-2 standards (log). The human FHR-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Related Products

Complement Factor H-Related Protein 3 (FHR-3) ELISA Assay Kit
Complement Factor H-Related Protein 4 (FHR-4) ELISA Assay Kit
Complement Factor H-Related Protein 5 (FHR-5) ELISA Assay Kit


Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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