Clostridium Difficile GDH ELISA Assay Kit
Clostridium Difficile GDH ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 Wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Stool
Sample Size: 250 mg
Alternative Names: C. Difficile, Fecal C. Difficile, Fecal Clostridium Difficile GDH, C. diff. Assay, C. diff ELISA, glutamate dehydrogenase
For Research Use Only
This Fecal Clostridium Difficile (C.diff) GDH ELISA is a “sandwich” ELISA that is designed, developed and produced for the quantitative and qualitative measurement of GDH in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.
Assay calibrators/controls and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal anti-GDH on its wall. During the assay, the GDH Antibody will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of GDH is added for further immunoreactions. After an incubation period, the immunocomplex of “Anti-GDH Capture Antibody – GDH – HRP-conjugated Anti-GDH Tracer Antibody” is formed if GDH is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to GDH proteins captured on the wall of each microtiter well is directly proportional to the amount of GDH level in each test specimen.