Chicken IgY ELISA Assay Kit

$935.00

The Chicken IgY ELISA Assay is designed for the quantitative determination of chicken IgY in complex samples (serum or other biological samples).  The Chicken IgY ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.
Chicken IgG and chicken IgY are two different names for the same antibody.  Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood.  It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them. IgY is often mislabelled as Immunoglobulin G (IgG) in older literature, and sometimes even in commercial product catalogues due to its functional similarity to mammalian IgG and Immunoglobulin E (IgE).

Chicken IgY ELISA Assay Kit

The Chicken IgY ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 80 ng/ml (sample dilution 1:25)
Dynamic Range:3.125 – 200 ng/ml
Incubation Time: 2.5 hours
Sample Type: Protein solutions, serum, biological fluids
Sample Size: 10 µL
Controls Included


Sample Preparation and Storage
Dilute the samples with diluent. If samples generate values outside the standard curve, the dilution factor may be varied. To exclude matrix effects the dilution factor should be at least 1:25.


Assay Background

Chicken IgG and chicken IgY are two different names for the same antibody. Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them. IgY is often mislabelled as Immunoglobulin G (IgG) in older literature, and sometimes even in commercial product catalogues due to its functional similarity to mammalian IgG and Immunoglobulin E (IgE).


Related Products

Chicken IgA ELISA Assay
Chicken SAA ELISA Assay
Chicken IgM ELISA Assay

Additional Information

Assay Principle


The determination of chicken IgY is carried out as direct sandwich ELISA. An antibody specific for chicken IgY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgY present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgY con­cen­tration.

Assay Procedure


A. Preparation
1.Bring all reagents to room temperature
2.Dilute wash buffer 1:10
3.Prepare the standard curve from a 1:2-dilution series of standard concentrate with diluent
4.Dilute samples with diluent

B. Performance
1.Pipette 100 µl of samples, standards, controls into the wells
2.Incubate for 1 hour at room temperature with shaking
3.Wash three times with 300 µl of wash buffer
4.Add 100 µl of HRP conjugate to each well
5.Incubate for 1 hour at room temperature with shaking
6.Wash three times with 300 µl of wash buffer
7.Dispense 100 µl of TMB substrate solution
8.Incubate for 10 minutes at room temperature in the dark
9.Add 100 µl of stop solution
10.Measure absorption at 450 nm

Typical Standard Curve


Manual

Product Manual


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